题干

Ⅰ.细菌常被应用于生产生活中,如有些细菌可分解原油,从而消除由原油泄漏造成的土壤污染.某同学欲从污染的土壤中筛选出能高效降解原油的细菌.

(1)在筛选过程中,应将土壤样品稀释液接种于以____ 为唯一碳源的固体培养基上.为筛选出高效菌株,可比较单菌落周围清晰区域的大小.一般情况下,如图中清晰区域越大说明____ .

Ⅱ.另一同学将第一步筛选得到的甲、乙、丙三种细菌分别接种到A、B、C三种培养液中,经培养后分别测得它们繁殖一代所需的时间,结果如表所示:

培养液成分

繁殖一代所需的时间(分钟)

A.葡萄糖、NaCl、PO43-、MgSO4

43

100

-

B.葡萄糖、NaCl、PO43-、MgSO4、8种氨基酸

28

40

-

C.葡萄糖、NaCl、PO43-、MgSO4、19种氨基酸

25

25

80

注:“﹣”表示细菌没有生长.

(2)在A培养基中增殖较快的细菌是____.丙细菌可以在C培养液中繁殖却无法在B培养液中繁殖,原因是____ 

(3)由于微生物的营养类型复杂,不同的微生物对营养物质的需求是不同的.如果培养能进行光合作用的微生物,培养基中的营养物质是____ (多选题),

A.碳源    B.氮源    C.生长因子    D.无机盐     E.水

Ⅲ.下面是有关某细菌G的四项实验:

实验1:将G接种于一般培养基上,结果出现菌落.

实验2:用一定剂量的紫外线处理G,产生突变种g1.将g1接种于一般培养基后,不出现菌落;但在培养基内添加营养物质甲后,就出现菌落.

实验3:用另一剂量的紫外线处理G,得突变种g2,将g2接种于一般培养基后,也不出现菌落;但在培养基内添加营养物质乙后,就出现菌落.

实验4:将g1和g2一起接种于一般培养基上,数日后出现菌落.

(4)细菌g1和g2分别接种于一般培养基,均不能生长.其原因是用紫外线处理导致细菌G发生了____ ,从而缺乏合成营养物质甲或乙的酶.

A.基因突变     B.基因重组     C.染色体畸变     D.不可遗传变异

(5)实验1至3表明,物质甲和乙均是细菌G在代谢过程中产生的代谢产物.在实验2和3 中,它们是作为____ 加入一般培养基的.

A.碳源     B.氮源        C.生长因子      D.无机盐

(6)实验4中出现菌落的原因可能是____ .

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同类题3

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    Maybe you are an average student. ____ This is not necessarily so, however. Anyone can become a better student if he or she wants to. Here's how.

    Plan your time carefully. When planning your work, you should make a list of things that you have to do. After making this list, you should make a schedule of your time. First your time for eating, sleeping, dressing, etc. Then decide a good, regular time for studying. ____ A weekly schedule may not solve all your problems, but it will force you to realize what is happening to your time.

    Make good use of your time in class. ____ Listening carefully in class means less work later. Taking notes will help you remember what the teacher says.

    Study regularly. When you get home from school, go over your notes; look at the important points that your teacher is going to discuss the next day, read that material. ____ If you do these things regularly, the material will become more meaningful, and you'll remember it longer.

    Develop a good attitude towards tests. The purpose of a test is to show what you have learned about a subject. They help you remember your new knowledge. The world won't end if you don't pass a test, so don't be over worried.

    ____ You will probably discover them after you have tried these.

A. There are other methods that might help you with your studying.

B. Don't forget to set aside enough time for relaxation.

C. Take advantage of class time to listen to everything the teacher says.

D. No one can become a top student unless he or she works hard.

E. You probably think you will never be a top student.

F. Make full use of class time to take notes of what the teacher says in class.

G. This will help you understand the next class.