运用下列各种细胞工程技术培育生物新品种，操作过程中能形成愈伤组织的是 
①植物组织培养  ②植物体细胞杂交  ③动物细胞培养 ④转基因植物的培养

阅读下面短文，然后从所给的四个选项中选出一个最佳答案。

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函数$y=\sin \left(\pi x+\phi \right)\left(\phi >0\right)$的部分图象如图所示,设P是 图象的最高点,A,B是图象与x轴的交点,记$\angle APB=\theta$,则$\sin 2\theta$的值是（　　）