人组织纤溶酶原激活物（htPA）是一种重要的药用蛋白，可在转htPA基因母羊的羊乳中获得．流程如图：

（1）htPA基因与载体用____ 切割后，通过DNA连接酶连接，以构建重组表达载体．检测目的基因是否已插入受体细胞DNA，可采用____ 技术．

（2）将重组表达载体导入受精卵常用的方法是____．

（3）若在转htPA基因母羊的羊乳中检测到____ ，说明目的基因成功表达．

已知函数$f\left(x\right)=\sqrt[3]{-x+1}$，则$\underset{△x\to 0}{\lim }\frac{f(1-∆x)-f\left(1\right)}{△x}$的值为（  ）

(A)

    Lisa is a teacher and her husband is a doctor. They are both very 1and have no time to do all the housework. So she asks her children to do some 2 according to their age. She asks John, the elder brother in the 3, to do the dishes. It is an easy chore 4 the 15-year-old boy. And she asks Dave, her 5 son, to take the dog for a walk every evening. The two boys also have to help 6 the car when it is dirty. And they clean their tables, 7 their beds every day and 8 food in the market every Sunday morning. The boys can cook easy meals 9 their parents are not at home.

    The family of 10 live in a big house 11they do all the housework by themselves. Lisa says, “When I was young, my father wanted us 12 with housework and learn how to 13 ourselves. Now I'm doing it to my kids.” John says, “Doing chores is 14.You can learn important life 15 and responsibility (责任).”

渭河平原属于（    ）