1.非选择题- (共1题)
（1）_____技术是单克隆抗体技术的基础。
（2）根据培养基的用途分类，图中培养基属于_____培养基。
（3）单克隆抗体与常规的血清抗体相比。最大的优越性是__________。
（4）动物细胞融合除了采用植物细胞原生质体融合常用诱导剂外，还可以采用__________。
（5）选出的杂交瘤细胞既具备骨髓瘤细胞的_____特点，又具备淋巴细胞的______特点。
（6）淋巴细胞是由动物体__________中的_________细胞分化、发育而来。上图中的某些淋巴细胞是指________。
（7）杂交瘤细胞从培养基中吸收葡萄糖、氨基酸的主要方式是_________。
2.单选题- (共5题)
| A．用温水使海藻酸钠迅速溶解，待其冷却到室温后用于包埋细胞 |
| B．进行包埋时，用于悬浮细胞的CaCl2溶液浓度要适宜 |
| C．注射器(或滴管)出口应尽量贴近液面以保证凝胶珠成为球状 |
| D．包埋酵母细胞的凝胶珠为淡黄色半透明状，并具有一定的弹性 |
| A．蒸馏法的实验原理是利用水将芳香油溶解下来，再把水蒸发掉，剩余的就是芳香油 |
| B．压榨法的实验原理是通过机械加压，压榨出植物的芳香油 |
| C．萃取法的实验原理是使芳香油溶解在有机溶解中，蒸发掉有机溶剂后就可以获得芳香油 |
| D．蒸馏法适用于提取玫瑰油、薄荷油等挥发性强的芳香油 |
| A．将抗原注入小鼠体内，获得能产生抗体的B淋巴细胞 |
| B．用纤维素酶处理B淋巴细胞与小鼠骨髓瘤细胞 |
| C．用聚乙二醇作诱导剂，促使能产生抗体的B淋巴细胞与小鼠骨髓瘤细胞融合 |
| D．筛选杂交瘤细胞，并从中选出能产生所需抗体的细胞群，培养后提取单克隆抗体 |
| | 植物细胞工程 | 动物细胞工程 |
| 特殊处理 | 酶解法去除细胞壁 | 胰蛋白酶处理配制细胞悬液 |
| 融合方法 | 物理方法，化学方法 | 物理方法，化学方法，生物方法 |
| 典型应用 | 人工种子，种间杂种植物 | 单克隆抗体的制备 |
| 培养液的区别 | 麦芽糖是离体组织赖以生长的成分 | 动物血清不可缺少 |
| A．0处 | B．1处 | C．2处 | D．3处 |
| A．动物细胞培养和植物组织培养所用培养基不同 |
| B．动物细胞培养和植物组织培养过程中都要用到胰蛋白酶 |
| C．烟草叶片离体培养能产生新个体，小鼠杂交瘤细胞可离体培养增殖 |
| D．动物细胞培养可用于检测有毒物质，茎尖培养可用于植物脱除病毒 |
3.选择题- (共2题)
“供给侧结构性改革”是2016年《政府工作报告》中的高频词汇，并且在2017年仍将占据我国经济生活的重要地位。正确的经济政策、适时的改革调整有利于经济的健康发展，39年前，做出改革开放这项正确决策的会议是（ ）
Leon was a postman. Every day after Leon woke up, he would feed his 37 cats. Leon didn't really want 37 cats, but one by one they had turned up on his doorstep, and Leon had taken them in.
Every day, after feeding the cats, Leon would collect the mail from the red postboxes in the village and take it home to sort（分类）. “If only I had 37 helpers instead of 37 cats, my job would be so much easier,” Leon thought. Just then, the cats started meowing for their dinner. That gave Leon an idea. That night, Leon stayed up making 37 cat-sized postal backpacks. Each backpack fitted neatly onto a cat's back. Then, he went around to each postbox, putting signs up that read: Postbox closed. Please use the Postal Cat Service.
The next day, Leon put a backpack on each cat and dropped off the cats around the village. Leon knew that the cats would be happy lazing around all day. He also knew that they would always come home for dinner. Leon spent the day relaxing, waiting for his helpers to return. Leon was right. The cats did return, but they did not bring home many letters. Somehow, the cats had managed to wriggle（蜿蜒而行） out of their backpacks. Or lose their letters. Or get the letters wet. “Cats are just too unreliable（不可靠的）” Leon thought. Just then, there was a scratching（刮擦声） at Leon's door. A dog was waiting to be invited inside. That gave Leon an idea.
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【1】题量占比
非选择题:(1道)
单选题:(5道)
选择题:(2道)
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【2】：难度分析
1星难题：0
2星难题：0
3星难题：0
4星难题：0
5星难题：0
6星难题：0
7星难题：0
8星难题：0
9星难题：6